SubtiBank SubtiBank
Version comparison:

2018-12-02 13:11:052025-05-27 22:01:16

locus

BSU40390

BSU_40390

geneLength

1365

1368

outlinks

bsu

BSU40390

BSU_40390

Gene

Coordinates

4,150,496 → 4,151,863

4,150,496 4,151,863

Gene

Phenotypes of a mutant

growth defect at high temperature, lysis at 49°C, this can be suppressed by mutations that reduce [[protein|WalK]] autokinase activity or by inactivation of [[gene|lytE]] [pubmed|29465029]

growth defect at high temperature, lysis at 49C, this can be suppressed by mutations that reduce [[protein|WalK]] autokinase activity, by inactivation of [[gene|lytE]], or by overexpreesion of [[protein|IseA]] or [[protein|PdaC]] [pubmed|29465029]

The protein

[SW|Localization]

cell membrane (according to Swiss-Prot), spotty close to the membrane [Pubmed|21219466]

cell membrane (according to UniProt), spotty close to the membrane [Pubmed|21219466]

Biological materials

Mutant

MGNA-B826 (yycH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1825 NBRP B. subtilis, Japan]

BKE40390 (Δ[[gene|walH]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE40390 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CGTAAGTAATATCGTTTTTA, downstream forward: _UP4_TTATTGAGGAAGGAGGGGGC

BKK40390 (Δ[[gene|walH]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK40390 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CGTAAGTAATATCGTTTTTA, downstream forward: _UP4_TTATTGAGGAAGGAGGGGGC

MGNA-B826 (yycH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1825 NBRP B. subtilis, Japan]

BKE40390 ([[gene|walH]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE40390 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CGTAAGTAATATCGTTTTTA, downstream forward: _UP4_TTATTGAGGAAGGAGGGGGC

BKK40390 ([[gene|walH]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK40390 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CGTAAGTAATATCGTTTTTA, downstream forward: _UP4_TTATTGAGGAAGGAGGGGGC